This bundle focuses on high-impact strategies to strengthen infection prevention by improving diagnostic practices, advancing antimicrobial stewardship, and eliminating low-value interventions. Sessions explore how diagnostic excellence serves as the foundation for effective healthcare epidemiology, with a particular emphasis on stewardship in special populations. Learners will gain insight into de-implementation—identifying and stopping practices that no longer add value—as well as practical approaches to diagnostic stewardship that enhance clinical decision-making and patient outcomes. The bundle also highlights sustainable actions that organizations can take now to drive meaningful, long-term improvements in infection prevention programs.